The combination of resveratrol and <i>Bletilla striata</i> polysaccharide decreases inflammatory markers of early osteoarthritis knee and the preliminary results on <scp>LPS</scp> ?induced <scp>OA</scp> rats

Osteoarthritis (OA) of the knee is characterized by progressive deterioration and loss articular cartilage with associatedstructural performance changes in entire joint, current treatments for OA only aim to relieve symptoms, rather than prevent or reverse disease progression. Recently, targeting “early osteoarthritis” (EOA) have attracted attention. However, during EOA stage, chondrocytes may change behaviors express pro-inflammatory cytokines free radicals, which would cause detrimental effects synovial cavity further wear. In this study, we combined resveratrol (Res) Bletilla striata polysaccharide (BSP) as anti-inflammatories antioxidants diffuse radicals alleviate inflammation from both short term long term. The study introduced a new method harvesting BSP as-received achieve high yields, shortened extraction times, maintained structure/functions. addition, it Res home-extracted (Res-BSP) oxidative stress Lipopolysaccharide (LPS)-induced model. gene expressions inflammatory genes iNOs, IL-1?, IL-6, MMP-13 were upregulated 5.7-fold, 6.5-fold, 8.6-fold, 4.5-fold, respectively on OA-like significantly downregulated 3.3-fold, 2.1-fold, 4.9-fold, 0.1-fold, respectively, once treated Res-BSP (p < 0.05, compared chondrocytes). chondrogenic TGF?1, SOX9, type II collagen 0.8-fold, 2.2-fold, based control group baseline. While was 3.4-fold, 0.32-fold, 0.4-fold, Res-BSP. Finally, elucidated role suppressing COX-2 activating p-Smad 2/3 p-Erk1/2. We believe that combination has great potential an alternative therapeutic strategy treatment future.